Identification of an infectious bronchitis coronavirus strain exhibiting a classical genotype but altered antigenicity, pathogenicity, and innate immunity profile
Identifieur interne : 000855 ( Main/Exploration ); précédent : 000854; suivant : 000856Identification of an infectious bronchitis coronavirus strain exhibiting a classical genotype but altered antigenicity, pathogenicity, and innate immunity profile
Auteurs : Shu-Yi Lin [Taïwan] ; Yao-Tsun Li [Taïwan] ; You-Ting Chen [Taïwan] ; Ting-Chih Chen [Taïwan] ; Che-Ming J. Hu [Taïwan] ; Hui-Wen Chen [Taïwan]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
Avian coronavirus infectious bronchitis virus (IBV) poses economic threat to the poultry industry worldwide. Pathogenic IBV 3575/08 was isolated from broilers vaccinated with the attenuated viral vaccine derived from a Taiwan strain 2575/98. In this study, extensive investigations were conducted on the genome sequences, antigenicity, pathogenicity, and host immune responses of several IBV strains in specific-pathogen-free chickens. Sequence analyses revealed that 3575/08 and 2575/98 shared high homology in their structural genes, but not in non-structural accessory proteins such as 3a, 3b and 5b. Despite a high degree of homology in their spike protein genes, cross neutralization test showed low cross protection between 3575/08 and 2575/98, suggesting distinct antigenicity for the two strains. Animal challenge experiments exhibited strong respiratory and renal pathogenicity for 3575/08. In addition, early and prolonged viral shedding and rapid viral dissemination were observed. Immune gene expression profiling by PCR array showed chickens infected with 3575/08 had delayed expression of a subset of early innate immune genes, whereas chickens infected with the wild-type or attenuated-type 2575/08 revealed quick gene induction and efficient virus control. In summary, this study reveals a new IBV strain, which harbors a known local genotype but displays remarkably altered antigenicity, pathogenicity and host defenses.
Url:
DOI: 10.1038/srep37725
PubMed: 27876864
PubMed Central: 5120290
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of an infectious bronchitis coronavirus strain exhibiting a classical genotype but altered antigenicity, pathogenicity, and innate immunity profile</title><author><name sortKey="Lin, Shu Yi" sort="Lin, Shu Yi" uniqKey="Lin S" first="Shu-Yi" last="Lin">Shu-Yi Lin</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Veterinary Medicine, National Taiwan University</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Li, Yao Tsun" sort="Li, Yao Tsun" uniqKey="Li Y" first="Yao-Tsun" last="Li">Yao-Tsun Li</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Veterinary Medicine, National Taiwan University</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, You Ting" sort="Chen, You Ting" uniqKey="Chen Y" first="You-Ting" last="Chen">You-Ting Chen</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Veterinary Medicine, National Taiwan University</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Ting Chih" sort="Chen, Ting Chih" uniqKey="Chen T" first="Ting-Chih" last="Chen">Ting-Chih Chen</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Veterinary Medicine, National Taiwan University</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Hu, Che Ming J" sort="Hu, Che Ming J" uniqKey="Hu C" first="Che-Ming J." last="Hu">Che-Ming J. 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Hu</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Institute of Biomedical Sciences, Academia Sinica</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Research Center for Nanotechnology and Infectious Diseases</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Hui Wen" sort="Chen, Hui Wen" uniqKey="Chen H" first="Hui-Wen" last="Chen">Hui-Wen Chen</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Veterinary Medicine, National Taiwan University</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Research Center for Nanotechnology and Infectious Diseases</institution>, Taipei,<country>Taiwan</country></nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Avian coronavirus infectious bronchitis virus (IBV) poses economic threat to the poultry industry worldwide. Pathogenic IBV 3575/08 was isolated from broilers vaccinated with the attenuated viral vaccine derived from a Taiwan strain 2575/98. In this study, extensive investigations were conducted on the genome sequences, antigenicity, pathogenicity, and host immune responses of several IBV strains in specific-pathogen-free chickens. Sequence analyses revealed that 3575/08 and 2575/98 shared high homology in their structural genes, but not in non-structural accessory proteins such as 3a, 3b and 5b. Despite a high degree of homology in their spike protein genes, cross neutralization test showed low cross protection between 3575/08 and 2575/98, suggesting distinct antigenicity for the two strains. Animal challenge experiments exhibited strong respiratory and renal pathogenicity for 3575/08. In addition, early and prolonged viral shedding and rapid viral dissemination were observed. Immune gene expression profiling by PCR array showed chickens infected with 3575/08 had delayed expression of a subset of early innate immune genes, whereas chickens infected with the wild-type or attenuated-type 2575/08 revealed quick gene induction and efficient virus control. In summary, this study reveals a new IBV strain, which harbors a known local genotype but displays remarkably altered antigenicity, pathogenicity and host defenses.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cook, J K" uniqKey="Cook J">J. K. Cook</name></author><author><name sortKey="Jackwood, M" uniqKey="Jackwood M">M. Jackwood</name></author><author><name sortKey="Jones, R C" uniqKey="Jones R">R. C. Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jackwood, M W" uniqKey="Jackwood M">M. W. 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Schmittgen</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Lin, Shu Yi" sort="Lin, Shu Yi" uniqKey="Lin S" first="Shu-Yi" last="Lin">Shu-Yi Lin</name></noRegion><name sortKey="Chen, Hui Wen" sort="Chen, Hui Wen" uniqKey="Chen H" first="Hui-Wen" last="Chen">Hui-Wen Chen</name><name sortKey="Chen, Hui Wen" sort="Chen, Hui Wen" uniqKey="Chen H" first="Hui-Wen" last="Chen">Hui-Wen Chen</name><name sortKey="Chen, Ting Chih" sort="Chen, Ting Chih" uniqKey="Chen T" first="Ting-Chih" last="Chen">Ting-Chih Chen</name><name sortKey="Chen, You Ting" sort="Chen, You Ting" uniqKey="Chen Y" first="You-Ting" last="Chen">You-Ting Chen</name><name sortKey="Hu, Che Ming J" sort="Hu, Che Ming J" uniqKey="Hu C" first="Che-Ming J." last="Hu">Che-Ming J. Hu</name><name sortKey="Hu, Che Ming J" sort="Hu, Che Ming J" uniqKey="Hu C" first="Che-Ming J." last="Hu">Che-Ming J. Hu</name><name sortKey="Li, Yao Tsun" sort="Li, Yao Tsun" uniqKey="Li Y" first="Yao-Tsun" last="Li">Yao-Tsun Li</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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